Please use this identifier to cite or link to this item: https://rda.sliit.lk/handle/123456789/1347
Full metadata record
DC FieldValueLanguage
dc.contributor.authorEeswera, J. P-
dc.contributor.authorPeiris, C. N-
dc.date.accessioned2022-02-22T09:31:39Z-
dc.date.available2022-02-22T09:31:39Z-
dc.date.issued1994-
dc.identifier.urihttp://rda.sliit.lk/handle/123456789/1347-
dc.description.abstractThe differences among cowpea (Vigna unguiculata (L) Walp) cultivars were identified using electrophoretically detected isozymic variability. The enzymes studied in the selected 10 cultivars of cowpea were Diaphorase (DIAP), Phospho Gluco Isomerase (PGI), 6Phospho Gluconate Dehydrogenase (6PGD), Phospho Gluco Mutase (PGM), Esterase (EST), Hexose Kinase (HK) and Glucose Oxalo Acetate Transaminase (GOT). Starch gel electrophoresis was used to analyze extracts prepared from young leaf tissues of seven day old seedlings of each cultivar grown under greenhouse conditions. Three enzymes GOT, HK, and PGM exhibited two patterns, while three different patterns were observed for EST and 6PGD. Phospho Gluco Isomerase showedfour different patterns, and DIAP appeared to be the most polymorphic with eight patterns. Distinct variety specific zymograms were observed for several enzymes. Unique combinations of isozymic variants of two enzyme systems assayed (DIAP and PGI) could differentiate all the culti*urs. Sufficient variability was present in cowpea cultivars to allow the use of isozyme electrophoresis as a method for cultivar identification. Therefore, ii could be used in variety identification to complement the morphological characters.en_US
dc.language.isoenen_US
dc.publisherPostgraduate Institute of Agriculture, University of Peradeniya: Peradeniyaen_US
dc.relation.ispartofseriesTropical Agricultural Research;Vol. 6 1994-
dc.subjectIdentificationen_US
dc.subjectCowpea Cultivarsen_US
dc.subjectVigna unguiculataen_US
dc.subjectIsoenzyme Electrophoresisen_US
dc.titleIdentification of cowpea cultivars (Vigna unguiculata (L) Walp) by isoenzyme electrophoresisen_US
dc.typeArticleen_US
Appears in Collections:Research Papers
Research Papers - School of Natural Sciences
Research Papers - SLIIT Staff Publications

Files in This Item:
File Description SizeFormat 
PGIATAR-6-185.pdf309.02 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.