Browsing by Author "Isaac, P.N."

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Improvement of the Survival Rate of In Vitro Propagated Dendrobium intermediate CV ‘White’ during Acclimatization
(Faculty of Humanities and Sciences - SLIIT, 2021-03-26) Isaac, P.N.; Peiris, S.E.
Dendrobium intermediate cv 'White', an ornamental plant with exquisite flowers which is produced through In vitro propagation, undergoes hyperhydricity in vitro conditions. As a result, they project a low survival rate during acclimatization which is a bottleneck for its success in micropropagation. This research aimed at overcoming the low survival rate during acclimatization of Dendrobium intermediate cv ‘White’ by improving the medium used in the pre- transplant stage. In vitro seedlings were cultured on Murashige & Skoog (MS) medium, without any growth regulators, with 3 sugar concentrations (3, 4 and 5% (w/v) at the pre-transplant stage for six weeks before being acclimatized in the protected house. After four weeks of acclimatization, the highest survival rate (100%), moderate survival rate (66.7 %) and lowest survival rate (12.5%) were observed in plants grown in vitro in MS medium fortified with 5, 4 and 3% (w/v) sugar concentrations respectively at the pre-transplant stage. Thus, increased sugar levels (5%, w/v) in the pre-transplant stage, can be recommended to achieve a 100% survival rate of Dendrobium intermediate cv ‘White’ during acclimatization. This is a promising solution to facilitate continuous mass production of Dendrobium intermediate cv ‘White’.