Browsing by Author "Kahatapitiya, N.S"

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Brewing plastics: OCT reveals microplastic release from nylon tea bags in simulated brewed tea infusions
(Royal Society of Chemistry, 2026-02-12) Jayasekara, P.M; Abhishek, P; Kahandawala,B.S; Damith, N; Weerasinghe, M; Kahatapitiya, N.S; Silva, B.N; Karunaratne, S; Wijesinghe, R.E; Wijenayake, U
The release of microplastics (MPs) from nylon tea bags poses a critical concern for human exposure; however,their detection and quantification remain challenging especially in beverage matrices, and hence, this study pioneers the use of high-resolution optical coherence tomography (OCT) integrated with an image processing algorithm to rapidly detect and quantify the size and count of the MPs directly in the water extractions simulating tea brewing. The water extractions prepared by simulating tea brewing conditions, hot (100 °C, 1–5min), cold (2 °C, 1 h), and ambient (30 °C, 1 h), were observed employing OCT imaging and validated through Nile Red (NR) staining and digital microscopy. The nylon tea bags steeped in hot water for 5 minutes released 16 000 to 24 000 LMPs (>30 mm) and SMPs (12–30 mm) per millilitre. The estimated daily intake (EDI) of MPs indicates a higher exposure for children (ranging from 0.201 to 0.349 mm3 kg−1 day−1 ) compared to adults (0.046 to 0.080 mm3 kg−1 day−1 ). In contrast, cold brewing for 1 hour released fewer LMPs but an equal quantity of small MPs (SMPs) compared to hot brewing. This OCT-based approach offers a rapid, versatile platform for the detection and quantification of MPs from diverse packaging materials and provides a powerful tool for comprehensive risk assessment when combined with chemical and toxicological analyses.
Open Access
Dense Convolutional Neural Network-Based Deep Learning Pipeline for Pre-Identification of Circular Leaf Spot Disease of Diospyros kaki Leaves Using Optical Coherence Tomography
(Multidisciplinary Digital Publishing Institute (MDPI), 2024-08) Kalupahana, D; Kahatapitiya, N.S; Silva, B.N; Kim, J; Jeon, M; Wijenayake, U; Wijesinghe, R. E
Circular leaf spot (CLS) disease poses a significant threat to persimmon cultivation, leading to substantial harvest reductions. Existing visual and destructive inspection methods suffer from subjectivity, limited accuracy, and considerable time consumption. This study presents an automated pre-identification method of the disease through a deep learning (DL) based pipeline integrated with optical coherence tomography (OCT), thereby addressing the highlighted issues with the existing methods. The investigation yielded promising outcomes by employing transfer learning with pre-trained DL models, specifically DenseNet-121 and VGG-16. The DenseNet-121 model excels in differentiating among three stages of CLS disease (healthy (H), apparently healthy (or healthy-infected (HI)), and infected (I)). The model achieved precision values of 0.7823 for class-H, 0.9005 for class-HI, and 0.7027 for class-I, supported by recall values of 0.8953 for class-HI and 0.8387 for class-I. Moreover, the performance of CLS detection was enhanced by a supplemental quality inspection model utilizing VGG-16, which attained an accuracy of 98.99% in discriminating between low-detail and high-detail images. Moreover, this study employed a combination of LAMP and A-scan for the dataset labeling process, significantly enhancing the accuracy of the models. Overall, this study underscores the potential of DL techniques integrated with OCT to enhance disease identification processes in agricultural settings, particularly in persimmon cultivation, by offering efficient and objective pre-identification of CLS and enabling early intervention and management strategies. © 2024 by the authors.
Open Access
Recent Technological Progress of Fiber-Optical Sensors for Bio-Mechatronics Applications
(MDPI, 2023-11-07) Abdhul Rahuman, M.A; Kahatapitiya, N.S; Amarakoon, V.N; Wijenayake, U; Silva, B.N; Jeon, M; Kim, J; Ravichandran, N.K; Wijesinghe, R.E
Bio-mechatronics is an interdisciplinary scientific field that emphasizes the integration of biology and mechatronics to discover innovative solutions for numerous biomedical applications. The broad application spectrum of bio-mechatronics consists of minimally invasive surgeries, rehabilitation, development of prosthetics, and soft wearables to find engineering solutions for the human body. Fiber-optic-based sensors have recently become an indispensable part of bio-mechatronics systems, which are essential for position detection and control, monitoring measurements, compliance control, and various feedback applications. As a result, significant advancements have been introduced for designing and developing fiber-optic-based sensors in the past decade. This review discusses recent technological advancements in fiber-optical sensors, which have been potentially adapted for numerous bio-mechatronic applications. It also encompasses fundamental principles, different types of fiber-optical sensors based on recent development strategies, and characterizations of fiber Bragg gratings, optical fiber force myography, polymer optical fibers, optical tactile sensors, and Fabry–Perot interferometric applications. Hence, robust knowledge can be obtained regarding the technological enhancements in fiber-optical sensors for bio-mechatronics-based interdisciplinary developments. Therefore, this review offers a comprehensive exploration of recent technological advances in fiber-optical sensors for bio-mechatronics. It provides insights into their potential to revolutionize biomedical and bio-mechatronics applications, ultimately contributing to improved patient outcomes and healthcare innovation.