International Conference on Sustainable Biotechnology [ICoSBi] 2025

Permanent URI for this collectionhttps://rda.sliit.lk/handle/123456789/4450

Browse

Filters

20252

Advanced Search

Filter by

Settings

Has files: YesSubject: search.filters.subject.green synthesis

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    PublicationOpen Access
    Effects of Green Synthesized Copper-Derived Nano Particles extracted from Piper betel to Eliminate Contaminations in tissue culture of Dracaena sanderiana Sander ex Mast. cv ‘Gold’
    (Department of Applied Sciences. Faculty of Humanities and Sciences,SLIIT, 2025-10-10) Gunawardana, R.; Peiris, S. E.; Peiris, C. N.; Jayasinghe, S.
    Micropropagation is often hindered by contamination from endophytic microbes. While antibiotics are used to eliminate these without harming plant tissues, they risk promoting antibiotic resistance. This study explores a green alternative by synthesizing copper nanoparticles (Cu-NPs) using antimicrobial-rich Piper betel leaf extract. The eco-friendly method uses plant biomolecules as natural reducing and stabilizingagents. The aim was to (i) produce Cu-NPs via eco-friendly plant-based synthesis, (ii) evaluate their antibacterial activity against Escherichia coli and S. aureus, and (iii) assess their effectiveness in reducing microbial contamination in plant tissue culture. Cu-NPs were produced by reacting leaf extract with cupric sulphate under controlled heating and stirring. Their antibacterial activity was tested against E. coli and Staphylococcus aureus via time-kill assays, and their efficacy in reducing contamination was assessed by applying them to Dracaena sanderiana explants in vitro. 200 ppm was identified as the optimal concentration for the antibacterial activity in in vitro establishment trials of Dracaena sanderiana, 50 ppm Cu-NPs proved to be the most effective concentration, resulting in 100% survival when used both for surface sterilization and as a supplement in the culture medium, and 80% survival when used only for surface sterilization. Thefindings suggest that green-synthesised Cu-NPs from P. betel show potential as an alternative to antibiotics in plant tissue culture, with promising effects on contamination control and reduced risk of antibiotic resistance.
  • Thumbnail Image
    PublicationOpen Access
    Reduced Graphene Oxide-Based Hybrid Electrospun Nanofibers Doped with Green- Synthesized Silver Nanoparticles: An Antibacterial Wound Healing Scaffold
    (Department of Applied Sciences. Faculty of Humanities and Sciences,SLIIT, 2025-10-10) Sithumini, A. G. P. M.; Rajapakshe, D. N.; De Silva, N.
    This research focuses on the development of novel antibacterial electrospun fibers composed of polyvinyl alcohol (PVA), hydrolyzed collagen (HC), and reduced graphene oxide (rGO), further modified with silver nanoparticles (AgNPs) for potential wound healing applications. Green synthesis of AgNPs, using a tea extract, was verified by a colour change from yellowish-brown to dark brown and the appearance of a UVVis absorbance peak at 450 nm. Scanning Electron Microscopy (SEM) revealed mostly spherical AgNPs with a primary size distribution between 10 and 50 nm. Energy Dispersive X-ray (EDX) analysis confirmed the presence of Ag atoms (a characteristic peak at 3 keV). Particle size analysis indicated an average AgNP size of 133 nm, with a particle size distribution showing a high-intensity peak at 299 nm and a lower-intensity peak at 18.39 nm, and a polydispersity index (PDI) of 0.471. SEM images of rGO-containing mats showed well-preserved fiber morphology with uniformly distributed AgNPs, in contrast to mats without rGO, where significant AgNP agglomeration covered fiber visibility. This suggests rGO's role in preventing nanoparticle aggregation and enhancing dispersion. Swelling ratio analysis demonstrated superior swelling in rGO-containing mats, reaching 390.48% after 48 hours compared to 273.6% for mats without rGO. The incorporation of rGO improves the antibacterial performance and mechanical properties of the scaffold, while green-synthesized nanoparticles further enhance the antibacterial efficacy.