In vitro Regeneration of Gypsophila Paniculata L. Through Nodal Segments

Abstract

Gypsophila (Gypsophila paniculata L.), commonly known as baby’s breath, is a very popular ornamental plant in the floriculture industry. Gypsophilas are often grown as ornamental plants in many European gardens. They are valuable as a cut flower in floristry to add as a filler to flower bouquets. A protocol for the regeneration of Gypsophila paniculata L. using nodal explants grown in the field was established. The induction of multiple shoots was best obtained on Murashige and Skoog (MS) medium supplemented with 1 mg L-1 BAP and 0.5 mg L-1 NAA. After 8 weeks of initial culture, multiple shoots were regenerated from the explants and transferred to the rooting medium with different concentrations of IBA and NAA. The highest number of roots was obtained from the MS medium supplemented with 0.5 mg L-1 IBA and 0.5 mg L-1 NAA within 6 weeks. Rooted plantlets were hardened and established in pots with 89% highest survival rate.