Green Synthesis, Characterizati on of Cu Nano Units and Evaluati on of Anti bacterial Properti es towards the Bacterial Blight Control in Anthurium andraeanum Lind under In-vitro and In-vivo Conditions

Abstract

Most types of metal nanoparti cles exhibit anti bacterial properti es. Copper nanoparti cles can generate reacti ve oxygen species (ROS) within bacterial cells, initi ati ng a cascade of events that lead to bacterial cell death. In this study, copper nano units (Cu NUs) were synthesized through green synthesis method using anthurium leaf extract toward the control of bacterial blight disease in Anthurium andraeanum cv ‘Rainbow’ plants. Synthesized Cu NUs were characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray diff racti on (XRD) techniques. Anti bacterial properti es of 30, 50 and 100 ppm of Cu NUs against Xanthomonas sp. which cause bacterial blight in anthurium plants were studied under invitro conditi ons using well diff usion test. Moreover, anti bacterial properti es of said NUs were studied at in-vivo levels by spraying Cu NUs at concentrati ons of 50, 100 and 200 ppm on bacterial blight infected anthurium cv ‘Rainbow. According to SEM and TEM analysis, synthesized NUs remained within size of 25.36 ± 12.55 nm length and 16.18 ± 3.75 nm width exhibiti ng a polygonal shape. In well diff usion test, opti mized 30 ppm concentrati on of Cu NUs formed an inhibiti on zone with a diameter of 20.6 ± 0.94 mm. In comparison, the anti bioti c sulfamethoxazole (positi ve control) formed an inhibiti on zone with a diameter of 26.3 ± 0.47 mm. At in-vivo level, 200, 100 and 50 ppm all three concentrati ons were able to control disease in 90% of treated plants within 5, 6 and 7 weeks respecti vely. Overall, this research highlights the potenti al of Cu NUs as an eff ecti ve alternati ve for controlling bacterial blight disease in anthurium cv ‘Rainbow’.