MacGregor, K. BShelp, B. JPeiris, SBown, A. W2022-02-172022-02-172003-09MacGregor, K.B., Shelp, B.J., Peiris, S. et al. Overexpression of Glutamate Decarboxylase in Transgenic Tobacco Plants Deters Feeding by Phytophagous Insect Larvae. J Chem Ecol 29, 2177–2182 (2003). https://doi.org/10.1023/A:10256509149470098-0331https://rda.sliit.lk/handle/123456789/1196Gamma-aminobutyrate (GABA) is a ubiquitous four-carbon, nonprotein amino acid synthesized by glutamate decarboxylase. Previous research suggests that the endogenous synthesis of GABA, a naturally occurring inhibitory neurotransmitter at neuromuscular junctions, serves as a plant resistance mechanism against invertebrate pests. In this study, two homozygous transgenic tobacco lines constitutively overexpressing a single copy of a full-length chimeric glutamate decarboxylase cDNA and possessing enhanced capacity for GABA accumulation (GAD plants), a homozygous transgenic line lacking the gene insert, and wild-type tobacco were employed. Tobacco budworm larvae were presented with plantattached wild type and transgenic leaves for 4 hr in a feeding preference study. Larvae consumed six to twelve times more leaf tissue from wild-type plants than from GAD plants. These results suggest that leaf GABA accumulation, which is known to occur in response to insect larval walking and feeding, represents a rapidly deployed localresistance mechanism.enGamma-aminobutyrateGABAglutamate decarboxylasephytophagousplant resistanceinsect larvaetransgenic plantsinvertebrate pestsArticlehttps://doi.org/10.1023/A:1025650914947