Isaac, P.N.Peiris, S.E.2022-01-072022-01-072021-03-26Isaac, P.N., and Peiris, S.E. (2020) Improvement of the Survival Rate of In Vitro Propagated Dendrobium intermediate CV ‘White’ during Acclimatization. Proceedings of SLIIT International Conference on Advancements in Sciences & Humanities:2783-8862https://rda.sliit.lk/handle/123456789/517Dendrobium intermediate cv 'White', an ornamental plant with exquisite flowers which is produced through In vitro propagation, undergoes hyperhydricity in vitro conditions. As a result, they project a low survival rate during acclimatization which is a bottleneck for its success in micropropagation. This research aimed at overcoming the low survival rate during acclimatization of Dendrobium intermediate cv ‘White’ by improving the medium used in the pre- transplant stage. In vitro seedlings were cultured on Murashige & Skoog (MS) medium, without any growth regulators, with 3 sugar concentrations (3, 4 and 5% (w/v) at the pre-transplant stage for six weeks before being acclimatized in the protected house. After four weeks of acclimatization, the highest survival rate (100%), moderate survival rate (66.7 %) and lowest survival rate (12.5%) were observed in plants grown in vitro in MS medium fortified with 5, 4 and 3% (w/v) sugar concentrations respectively at the pre-transplant stage. Thus, increased sugar levels (5%, w/v) in the pre-transplant stage, can be recommended to achieve a 100% survival rate of Dendrobium intermediate cv ‘White’ during acclimatization. This is a promising solution to facilitate continuous mass production of Dendrobium intermediate cv ‘White’.enhyperhydric5% (w/v) sugaracclimatizationpre-transplant stageArticle