Browsing by Author "Nagahatenna, D. S. K"

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    PublicationOpen Access
    In vitro propagation of Hemidesmus indicus (L) R. Br.(Iramusu) through nodal culture
    (Postgraduate Institute of Agriculture, University of Peradeniya: Peradeniya, 2007) Nagahatenna, D. S. K; Peiris, S
    he effects of different nodal positions and 6-benzylaminopurine (BAP) concentrations on shoot development from nodal explants of Hemidesmus indicus (L.) Br.(Iramusu) were investigated. The highest number of shoots per explant (2.57±0.97) was observed in 4th nodal position, while the longest shoot length (3.23±0.52 cm) was achieved in 5th nodal position at 2 mg/L BAP with 0.1 mg/L Naphthalene Acetic Acid (NAA). Reduction of total shoot length and number of shoots were observed when BAP concentration was gradually increased from 5 to 15 mg/L. Half-strength semi solid MS medium with 1.5 mg/L indole-3-butyric acid (IBA) exhibited the best in vitro rooting. Hundred percent of the rooted shoots survived during the acclimatization.
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    PublicationOpen Access
    Modification of plant architecture of Hemidesmus indicus (L.) R. Br.(Iramusu) by in vitro colchicine treatment
    (Postgraduate Institute of Agriculture, University of Peradeniya: Peradeniya, 2008) Nagahatenna, D. S. K; Peiris, S
    he effects of different concentrations of colchicine on shoot and root development of nodal explants and germinating somatic embryos of Hemidesmus indicus (L.) R. Br.(Iramusu) was investigated. MS medium solidified with 8 g/l agar was superior to the liquid medium in all the concentrations of colchicine. In nodal derived H. indicus plants, the highest number of shoots per explant (4.23±1.49) and the longest shoot length (6.36±4.29 cm) were achieved at 5 mg/l colchicines forming a compact bushy type plant architecture. In almost all the treatments an increment of shoot length and number of shoots per explant could be observed compared to the control plants. Furthermore, 1 mg/l colchicine was more effective in germinating somatic embryos to produce more compact plant type under in vitro condition. There was no significant difference observed in different concentrations of colchicine on root length and root …
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    PublicationOpen Access
    Plant Regeneration System for Osbeckiaoctandra (L.) DC: A Valuable Medicinal Plant
    (Sri Lanka Institute of Information Technology, 2020-12-01) Nagahatenna, D. S. K; Peiris, S
    Osbeckiaoctandra (HeenBovitiya), which is one of the most valuable ayurvedic medicinal and ornamental plants in Sri Lanka, is now threatened due to its overexploitation from their natural habitat. In order to produce high quality, disease-free and genetically identical plant materials in large scale, we developed a highly efficient in vitro clonal propagation system using leaf explants. The effects of three different concentrations of plant growth regulators (6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA), kinetin and incubation conditions on plant regeneration were investigated. Plant growth parameters were analyzed in 15 biological replicates using one-way ANOVA. Present study revealed that the highest number of shoots per leaf explant with 92.4% shoot induction rate was achieved when young mature leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 3 mg/L BAP and 0.5 mg/L NAA and incubated under dark conditions. When microshoots were separated and subcultured onto hormone-free MS medium containing high sugar content (4%), rapid shoot multiplication and a vigorous root development was detected under light conditions. The in vitro grown plantlets were successfully acclimatized and 89% of the regenerated plantlets survived. Our novel clonal propagation system will open new avenues for mass propagation of O. octandra plants for the pharmaceutical industry and improving their medicinal and ornamental characteristics through biotechnological tools