Research Papers - School of Natural Sciences

Permanent URI for this collectionhttps://rda.sliit.lk/handle/123456789/1352

Browse

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    PublicationOpen Access
    Efficient low cost seesap (CSUP) technique for micropropagation of newly introduced sweetener plant, Stevia rebaudiana Bertoni
    (Academic Journals, 2019) Peiris, S; Lankika, P. C; Thilakarathne, P. L
    Stevia rebaudiana is a non-caloric sweetening herb, which has a high potential for commercialization. During this study low cost media sterilization, seesap (CSUP) technique which applies sodium hypochlorite was used as an alternative to autoclaving. In vitro cultures were established using nodal segments collected from stevia mother plants. These in vitro shoots were cultured on Murashige & Skoog (MS) medium supplemented with 16 combinations of 6- benzyl amino purine (BAP) and kinetin (KN) with 3% sugar at 5.8 pH, to investigate the in vitro shoot multiplication. The developed shoots were transferred to half MS medium supplemented with 0, 1 and 2 mg/L Indole butyric acid (IBA) with 3, 4 and 5% sugar in nine combinations at 5.8 pH, to investigate the rooting. The well rooted stevia plantlets were transferred to Jiffy™ bags for hardening. The results showed that the combination of 1.0 mg/L BAP and 1.5 mg/L KN resulted in the highest number of shoots (11.8 shoots/shoot). The combination of 2.0 mg/L IBA with 5% sugar in half MS medium resulted in the highest root induction (7.2 roots / plant) and root elongation (1.02 cm). The plants grown in culture medium containing 5% sucrose gave 100% survival rate at acclimatization.
  • Thumbnail Image
    PublicationEmbargo
    Improvement of the Survival Rate of In Vitro Propagated Dendrobium intermediate CV ‘White’ during Acclimatization
    (Faculty of Humanities and Sciences - SLIIT, 2021-03-26) Isaac, P.N.; Peiris, S.E.
    Dendrobium intermediate cv 'White', an ornamental plant with exquisite flowers which is produced through In vitro propagation, undergoes hyperhydricity in vitro conditions. As a result, they project a low survival rate during acclimatization which is a bottleneck for its success in micropropagation. This research aimed at overcoming the low survival rate during acclimatization of Dendrobium intermediate cv ‘White’ by improving the medium used in the pre- transplant stage. In vitro seedlings were cultured on Murashige & Skoog (MS) medium, without any growth regulators, with 3 sugar concentrations (3, 4 and 5% (w/v) at the pre-transplant stage for six weeks before being acclimatized in the protected house. After four weeks of acclimatization, the highest survival rate (100%), moderate survival rate (66.7 %) and lowest survival rate (12.5%) were observed in plants grown in vitro in MS medium fortified with 5, 4 and 3% (w/v) sugar concentrations respectively at the pre-transplant stage. Thus, increased sugar levels (5%, w/v) in the pre-transplant stage, can be recommended to achieve a 100% survival rate of Dendrobium intermediate cv ‘White’ during acclimatization. This is a promising solution to facilitate continuous mass production of Dendrobium intermediate cv ‘White’.