SLIIT International Conference on Advancements in Sciences and Humanities [SICASH] 2022
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Publication Embargo Effects of Coconut Water on Micropropagation of Caladium Bicolour Cv ’Thai Beauty’(Faculty of Humanities and Sciences, SLIIT, 2022-09-15) Ratnayake, R.D; Peiris, S.E; Malwattage, G; Peiris, C.NCaladium bicolour (Aiton) Vent. is an attractive commercial ornamental plant in the horticulture industry which is popular as potted plants for homescaping and as garden plants for landscaping. Propagation of this attractive plant through micropropagation has more benefits than conventional propagation. The current study describes an in vitro multiplication of Caladium bicolour cv ‘Thai Beauty’ with coconut water as a supplement. Shoots derived from leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1 and 1.5 mg/L (6- Benzylaminopurine) with and without 120 mL/L of coconut water. The results showed that coconut water (CW) with 0.5 and 1.5 mg/L BAP in the medium increased caladium shoot multiplication having an average of 6.67 and 7.75 shoots /shoot, respectively, with a large number of high-quality shoots. Coconut water alone in the medium also produced average of 3.42 shoots/ shoot. Hence, the current protocol provides a direct, cost-effective mass propagation method for large-scale commercial cultivation of caladium.Publication Embargo In Vitro Antibacterial Activity of Sulphur Nanoparticles as a Possible Application to Control Bacterial Blight Caused by Xanthomonas spp. in Anthurium(Faculty of Humanities and Sciences, SLIIT, 2022-09-15) Peiris, S.E; Malwattage, G; Ratnayake, R. D; Seneviratne, K .L; Peiris, C.NAnthurium blight is caused by Xanthomonas spp. which is regarded as the most threatening disease to the anthurium industry worldwide. Therefore, the current study was carried out to determine whether the application of sulphur nanoparticles (SNPs) is a possible solution for treating anthurium cultivars infected with Xanthomonas spp. The bacterium Xanthomonas was isolated using standard methods and a single bacterial colony was isolated using nutrient agar. The colonies were identified as Xanthomonas spp as they were gram-negative, motile rods due to the colony characters like yellow color because of the xanthin produced. The symptoms appeared in the pathogenicity test which was carried out by injecting purified Xanthomonas sp. into disease free anthurium plants confirmed the identification of the bacterial strain. Time-kill assay was conducted using Staphylococcus aureus, Escherichia coli and isolated Xanthomonas spp to investigate the behavior of SNPs. The results showed that suspension treated with 1g of SNPs for 30 minutes inhibited growth of Staphylococcus aureus colonies showing mean number of 7.92 CFU/ml compared to the control (mean number of colonies 9.09 CFU/ml ) treatment following 12 hours incubation. However, Escherichia coli, and isolated gram-negative rods (Xanthomonas spp) did not show positive influence for SNPs when compared to the control treatment. Therefore, further investigation is required to reach firm conclusions about this matter because the antimicrobial activity of SNPs varies depending on the type of target microorganisms, method and solvent used to dissolve SNPs.