Research Papers - Department of Mechanical Engineering

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    Co-production of fucoxanthin, docosahexaenoic acid (DHA) and bioethanol from the marine microalga Tisochrysis lutea
    (Elsevier, 2021-12-01) Premaratne, M; Liyanaarachchi, V. C; Nimarshana, P. H. V; Ariyadasa, T. U; Malik, A; Attalage, R. A
    The marine microalga Tisochrysis lutea is renowned for its ability to synthesize fucoxanthin and docosahexaenoic acid (DHA), which are nutritionally valuable high-value compounds. Although numerous studies in literature have assessed fucoxanthin and DHA production by T. lutea, very few studies have evaluated the feasibility of comprehensively utilizing biomass for co-production of these metabolites within the framework of biorefineries. To this end, the current study focused on the synthesis of fucoxanthin and DHA by cultivation of T. lutea under two different initial nitrate concentrations (1x: 882 µM, 3x: 2,646 µM) and three different light intensities (LL: 50 µmol/m2/s; ML: 100 µmol/m2/s; HL: 150 µmol/m2/s). The maximum fucoxanthin yield of 8.80 ± 0.30 mg/L (14.43 ± 0.52 mg/g) and DHA yield of 7.08 ± 0.02 mg/L (11.90 ± 0.14 mg/g) were achieved in the 3x HL culture at the end of 16 days of cultivation. Thereafter, a novel process of biphasic solvent extraction using ethanol/n-hexane/water (10:9:1 v/v/v) was utilized for co-extraction 97.96 ± 0.54% fucoxanthin and 74.11 ± 1.49% DHA from 3x HL biomass, and products were separated into two fractions. Fermentation of the residual biomass obtained from co-extraction resulted in a bioethanol yield of 48.49 ± 0.58 mg/g. Accordingly, the current study demonstrated the potential of T. lutea as a feedstock for biorefineries.
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    PublicationOpen Access
    Comparative assessment on the extraction of carotenoids from microalgal sources: Astaxanthin from H. pluvialis and β-carotene from D. salina
    (Elsevier, 2019-03-20) Rammuni, M N; Ariyadasa, Thilini U; Nimarshana, P. H. V; Attalage, R. A
    Astaxanthin and β-carotene are important carotenoids used in numerous pharmaceutical and nutraceutical applications, owing to their vigorous antioxidant properties. The microalgal strains Haematococcus pluvialis and Dunaliella salina accumulate the highest quantities of astaxanthin and β-carotene (up to 7% and 13% dry weight respectively) and are therefore considered as sustainable feedstock for the commercial production of carotenoids. Thus, from an economical perspective, it becomes desirable to optimize recovery of carotenoids from microalgal cells. To this end, here, we have summarized the conventional and modern extraction techniques generally used for the recovery of astaxanthin from Haematococcus pluvialis and β-carotene from Dunaliella salina. Furthermore, we have also discussed the optimum process conditions employed for numerous extraction protocols including solvent extraction, ultrasonic-assisted extraction (UAE), microwave-assisted extraction (MAE) and supercritical fluid extraction (SFE). Overall, our study highlights the sustainability of integrated co-production of biofuels and carotenoids in a biorefinery framework.