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    Visible Light Active Silver Decorated Iron Titanate/Titanium Dioxide Nanohybrid for Sterilization of Explants Grown by In Vitro Technique
    (John Wiley and Sons, 2022-12-04) Seneviratne, K .L; Munaweera, I; Peiris, S. E; Kodithuwakku, P; Peiris, C. N; Kottegoda, N
    Visible light (VL) active silver decorated iron titanate/titanium dioxide (Ag-FeTiO3/TiO2) nanohybrids derived from natural ilmenite sand effectively cleanse the exophytic plant pathogens in explants grown by in vitro techniques. VL active nanohybrid is synthesized by acid-hydrolysis of natural ilmenite, followed by precipitation of the lixivium and decoration with Ag onto FeTiO3/TiO2 nanoparticles, and finally calcination under a nitrogen environment to obtain Ag-FeTiO3/TiO2. A morphological study conducted using transmission electron microscopy (TEM) confirms the formation of Ag-FeTiO3/TiO2. Powder X-ray diffraction (PXRD) analysis shows that the nanohybrid primarily consists of anatase, iron titanate (FeTiO3), Ag2O, and Ag. X-ray photoelectron spectroscopy (XPS) study suggests the presence of Ag0/Ag2O/Fe2O3 composites on the FeTiO3/TiO2 particle surface. The optical band gap significantly changes from 3.14 eV (ilmenite) to 2.80 eV after the decoration of FeTiO3/TiO2 with Ag. This nanohybrid is utilized as a surface-sterilizing agent for in vitro establishment of the Dracaena sanderiana Sander ex Mast plant. Findings illustrate Ag-FeTiO3/TiO2 nanohybrid-based photo-sterilization leads to the survival of 90% of the microbes’ free cultures while the material can be re-used due to its photocatalytic behavior. Thus, the newly synthesized nanohybrid can replace harmful sterilization agents used in tissue culture techniques