SLIIT Conference and Symposium Proceedings
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All SLIIT faculties annually conduct international conferences and symposiums. Publications from these events are included in this collection.
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Publication Embargo Effects of Coconut Water on Micropropagation of Caladium Bicolour Cv ’Thai Beauty’(Faculty of Humanities and Sciences, SLIIT, 2022-09-15) Ratnayake, R.D; Peiris, S.E; Malwattage, G; Peiris, C.NCaladium bicolour (Aiton) Vent. is an attractive commercial ornamental plant in the horticulture industry which is popular as potted plants for homescaping and as garden plants for landscaping. Propagation of this attractive plant through micropropagation has more benefits than conventional propagation. The current study describes an in vitro multiplication of Caladium bicolour cv ‘Thai Beauty’ with coconut water as a supplement. Shoots derived from leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1 and 1.5 mg/L (6- Benzylaminopurine) with and without 120 mL/L of coconut water. The results showed that coconut water (CW) with 0.5 and 1.5 mg/L BAP in the medium increased caladium shoot multiplication having an average of 6.67 and 7.75 shoots /shoot, respectively, with a large number of high-quality shoots. Coconut water alone in the medium also produced average of 3.42 shoots/ shoot. Hence, the current protocol provides a direct, cost-effective mass propagation method for large-scale commercial cultivation of caladium.Publication Embargo An Efficient Plant Regeneration Using Leaf Explant for Osbeckia octandra (L.) DC; A Valuable Medicinal and Ornamental Plant(Faculty of Humanities and Sciences - SLIIT, 2021-03-26) Dilrukshi, S. K.; Nagahatenna, D.S.K.; Peiris, Sriyani E.Osbeckia octandra (Heen Bovitiya), which is one of the most valuable ayurvedic medicinal and ornamental plants in Sri Lanka is now threatened due to overexploitation in its natural habitat. In order to preserve this economically important plant species, a highly efficient in vitro clonal propagation system using leaf explants was developed in this study. The effects of three different concentrations of plant growth regulators: (6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA) and kinetin) and incubation conditions on plant regeneration were investigated. The plant growth parameters were analyzed in 15 biological replicates using one- way or two-way ANOVA. The present study revealed that the highest number of shoots per leaf explant with 92.4% shoot induction rate was achieved when young mature leaf explants were inoculated on Murashige and Skoog (MS) medium supplemented with 3 mg/L BAP and 0.5 mg/L NAA under dark conditions. When micro shoots were separated and subcultured on hormone-free MS medium with high sugar content (4%), shoots multiplied rapidly and a vigorous root system was developed under light conditions. The in vitro grown plantlets were successfully acclimatized and 89% of the regenerated plantlets survived. Our novel clonal propagation system will open new avenues for large- scale multiplication and improvement of medicinal and ornamental characteristics of this valuable species using biotechnological tools.Publication Embargo Production of Disease Free Rumohra adiantiformis (Leatherleaf Fern) Using In Vitro Propagation(Faculty of Humanities and Sciences - SLIIT, 2021-03-26) Perera, D.; Peiris, S.E.This study investigated the use of rhizome tips as explants in regenerating disease-free plantlets of Rumohra adiantiformis (G. Frost) using the micropropagation technique. Rhizome tips of 0.5 cm, 1 cm, 1.5 cm, and 2 cm in length, and with and without outer skin were used as explants.The highest regeneration rate (30%) was achieved with an explant size of one centimetre when the outer skin was not present in rhizome explants. Rhizome tips produced complete plantlets in Murashige and Skoog (MS) medium without any growth regulators in 80 - 90 days of culture initiation. In vitro multiplication of disease-free plants was achieved by culturing four months old in vitro plantlets in the MS medium supplemented with 2 mg/L Benzyl Amino Purine (BAP) and 0.1mg/L Naphthalene Acetic Acid (NAA). Plantlets produced clusters of shoot primordia which are known as green globular bodies (GGB) in the multiplication medium, 10 weeks after culturing. Plantlets were obtained by culturing 2-3 mm size GGB segments in the basal medium without any plant growth regulators. Disease indexing of the in vitro derived plantlets verified that at in vitro level 60% of the plantlets obtained from the rhizome tips of one centimetre were free from bacterial and fungal diseases.