Faculty of Humanities and Sciences
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Publication Open Access A Novel Surface Sterilization Technique for in vitro Establishment of Dianella tasmanica variegata Nodal Explants(researchgate.net, 2021-12) Malwattage, G; Ratnayake, R. D; Seneviratne, K. L; Peiris, S.E; Peiris, B. C. NDianella tasmanica ‘variegata’ is one of the popular species which is exported as ex-agar plants from Sri Lanka. In order to promote dianella exports, micropropagation should be increased to produce a high-quality large number required by the export markets. However, severe microbial contaminations in the in vitro establishment have become the bottle neck for large scale in vitro propagation of this species. Therefore, this study was undertaken with the objective of using sulfur nanoparticles (S-NP) to eliminate surface adhered fungal and bacterial contaminants to obtain a vast number of contamination-free cultures at the in vitro establishment stage. Apical parts of about 6 cm of D. tasmanica were used in this study. Effects of S-NP solution in 500 mg/L was used with the control of 10% Clorox™ for the surface sterilization of the nodal explants. Results revealed that 500 mg/L S-NP produced 80% and CloroxTM produced 40% contamination-free cultures after 4 weeks of establishment in vitro. The experiment was repeated twice. This study suggests that S-NP is a promising lowcost non-toxic material that can be used in the surface sterilization of dianella nodal explants.Publication Open Access Biodiversity of fruit and other tree species in the Huruluwewa watershed with emphasis on their economic utility(Postgraduate Institute of Agriculture, University of Peradeniya: Peradeniya, 1997) Amarasinghe, A. A. Y; Peiris, B. C. N; Peiris, SThis stud}1 attempted to determine species and genetic diversity in the Huruluwewa wa'ershed considering basic information on native tree species with emphasis oi i fruit and other tree species of future economic utility. This study :vas carried out in different phases, namely, "reconnaissance survey" leading to specific information of the Huruluwewa watershed to determine the species diversity and "Identification of genetic diversity of fruit tree species" through starch gel electrophoresis techniques. Few groups of fruit tree species showing close similarities were used to establish the "Finger printing" through starch gel electrophoresis to identify the genetic diversity. The existing high degree of species diversity in the Huruluwewa watershed can be categorized into different groups based on their economic utility. These are fruit ti ee species, medicinal plants, firewood species, timber trees, ornamental plants food crops, shade trees, cover crops andfodder crops. Different banding patterns in the starch gel electrophoresis established for fruit tree species with closn morphological similarities, indicated the genetic diversity within and among fruit tree species in the watershedPublication Open Access Effects of selected post harvest treatments on vase life of cut flower gladiolus (Gladiolus grandiflorus)(Postgraduate Institute of Agriculture, University of Peradeniya: Peradeniya, 2000) Premawardena, P. S; Peiris, B. C. N; Peiris, SA postharvest storage study with different treatments was carried out to investigate a procedure in prolonging the vase life of cut-flower gladiolus. Various chemical and non chemical treatments which are locally available were used. In comparison with the control (distilled water), beverages such as 7-UP™ in different concentrations, specific anti-ethylene compounds, silver nitrate and silver thiosulphate (STS), benzyl amino purine (BAP) in different concentrations, hot water treatment to the base ofthe flower stalk as a sterilizing method, maintaining the pH at 3.5 using HCI, eylene oxidization with KMn04 and ethylene absorption with activated charcoal were tested The longest vase-life and the best visual quality were achieved by gladiolus flowers treated with 25% 7-UP™. Next best visual quality were achieved by Silver nitrate, 2.25% 7-UP™, 4 mg t' KMn04 and STS in the descending order. Flowers treated with 2 mg t' KMn04 and the control (distilled water) had the lowest vase life. The visual quality was lowest in flowers treated with 2 mg t1 KMnO^ When the cots ofcompounds used were considered the study indicates that the treatment 7-UP™ and KMn04 were found to be the cheapest, while silver nitrate and silver thiosulphate are comparatively expensive.Publication Open Access Potential low cost treatments for extending the vase life of anthurium (Anthurium andreanum lind) flowers(Postgraduate Institute of Agriculture, University of Peradeniya: Peradeniya, 2000) Yapa, S. S; Peiris, B. C. N; Peiris, SFlowers, being viable and actively metabolizing parts of plants, are subject to ageing processes and thus are perishable commodities. In order to meet the requirement for export market of cut-flowers it is necessary to prolong the vase-life while maintaining quality. Specific anti- ethylene compounds, which are expensive, are presently being used to break the ageing process. An investigation was therefore carried out to extend the vaselife of cut-flower Anthurium gndregnum. using inexpensive treatments in comparison with conventional methods. The flowers were treated with different concentrations of antiethylene compounds, such as AgNO, silver thiosulfate (STS), benzyl amino purine (BAP), KMn04 , glycerine and hot water. Distilled water treatment was used as the control. The longest vase-life lasting 14 days was obtained with 2 mg t' KMnO+ This was followed by STS (12.87days), 10 mg t' BAP (12.43 days), 5 mg t1 BAP( 12.37 days) and 1000 mg t' AgN03 (12.25 days). Hot water and 15 mg t' BAP did not significantly affect the vase-life. Flowers treated with different glycerine concentrations showed the lowest- vase-life compared to the control. The cost analysis revealed that the KMn04 treatment is the cheapest method. Moreover, amongst conventional treatments, AgNO3, BAP and STS at tested concentrations were not found to be cost effective.Publication Open Access CSUP technique: a low cost sterilization method using sodium hypochlorite to replace the use of expensive equipment in micropropagation(National Science Foundation of Sri Lanka, 2012-03-28) Peiris, S; De Silva, E. D. U. D; Edussuriya, M; Attanayake, A. M. U. R. K; Peiris, B. C. NThe ability of in vitro micropropagtion to produce high quality planting material in large quantities is hindered by their high cost of production. Hence the price of planting material is high, making them unaffordable to growers. However, a larger portion of the capital and running cost of micropropagation can be reduced using CSUP (pronounced as seesap) technique, where sodium hypochlorite is used to sterilize glassware and culture media followed by culturing without using a laminar flow cabinet. This study was undertaken with the objective of evaluating the feasibility of the CSUP technique to replace the sterilization carried out using autoclaves and laminar flow cabinets. Sodium hypochlorite in four concentrations 5, 10, 15 and 20% (v/v) were used to rinse glassware and accessories prior to pouring the appropriate culture medium for anthurium cv ‘Tropical Red’ in vitro shoot multiplication. The culturing of axenic shoots was carried out inside a glass-cage and cultures were placed in the growth room. Autoclaved culture medium and a laminar flow cabinet were used in the case of the control cultures. Results revealed that the percentage of contaminationfree cultures, dry weight increase and the shoot multiplication were not significantly different between the respective treatments and the controls, as well as among treatments of different sodium hypochlorite concentrations. Therefore, the CSUP technique using 5% (v/v) sodium hypochlorite solution can be adopted to replace the use of an autoclave and the laminar flow cabinet in the major micropropagation activity of in vitro multiplication.