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Author: search.filters.author.Wanaguru, S

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    PublicationOpen Access
    Copper-Based Nanoparticles as Precision Nanoantibiotics against Bacterial Blight in Anthurium andraeanum Lind.: Synthesis, Characterization, and Antibacterial Efficacy under In Vitro and In Vivo Conditions
    (American Chemical Society, 2026-05-05) Wanaguru, S; Jayasinghe, S; Seneviratne, K; Kottegoda, N; Peiris, Sriyani E; Peiris, Colin N
    The bacterial blight disease caused by Xanthomonas spp., a dilemma for farmers worldwide. The existing conventional methods have failed to effectively combat the disease. Therefore, it is crucial to focus on developing and implementing more affordable techniques to effectively manage bacterial blight effectively. Herein, we investigated the efficacy of copper-based nanoparticles (Cu NPs) synthesized via three different methods toward controlling bacterial blight disease in Anthurium andraeanum plants. For that, Cu NPs were engineered via chemical, semichemical, and green synthesis approaches. Cu NPs were characterized through scanning electron microscope (SEM), transmission electron microscope (TEM), Fourier-transform infrared (FTIR), and powder X-ray diffraction (PXRD) analysis. Quantitative antibacterial assays revealed that the chemical and green-synthesized Cu NPs exhibited the highest potency, with a minimum inhibitory concentration (MIC) of 31.25 μg/mL and a minimum bactericidal concentration (MBC) of 62.50 μg/mL, whereas the semichemical formulation showed lower activity (MIC = 62.50 μg/mL; MBC = 125 μg/mL). In vitro well-diffusion assays further supported this trend: at 30 ppm, the inhibition zones measured 25 ± 0.81, 15 ± 0.81, and 20.6 ± 0.94 mm for the chemical, semichemical, and green synthesis methods, respectively, compared to 26.3 ± 0.47 mm for the positive control-antibiotic sulfamethoxazole. At in vivo, optimized concentration of 200 ppm, Cu NPs produced via all three methods controlled bacterial blight disease in 90% of treated anthurium plants within 4, 7, and 5 weeks, respectively, without causing phytotoxicity. Disease progression was monitored over 12 weeks by measuring lesion areas, and statistical significance of treatments was determined using two-way analysis of variance (ANOVA). ©
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    PublicationOpen Access
    Green Synthesis, Characterizati on of Cu Nano Units and Evaluati on of Anti bacterial Properti es towards the Bacterial Blight Control in Anthurium andraeanum Lind under In-vitro and In-vivo Conditions
    (Faculty of Humanities and Sciences, SLIIT, 2024-12-04) Wanaguru, S; Peiris, C.N; Peiris, S.E; Jayasinghe, S
    Most types of metal nanoparti cles exhibit anti bacterial properti es. Copper nanoparti cles can generate reacti ve oxygen species (ROS) within bacterial cells, initi ati ng a cascade of events that lead to bacterial cell death. In this study, copper nano units (Cu NUs) were synthesized through green synthesis method using anthurium leaf extract toward the control of bacterial blight disease in Anthurium andraeanum cv ‘Rainbow’ plants. Synthesized Cu NUs were characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray diff racti on (XRD) techniques. Anti bacterial properti es of 30, 50 and 100 ppm of Cu NUs against Xanthomonas sp. which cause bacterial blight in anthurium plants were studied under invitro conditi ons using well diff usion test. Moreover, anti bacterial properti es of said NUs were studied at in-vivo levels by spraying Cu NUs at concentrati ons of 50, 100 and 200 ppm on bacterial blight infected anthurium cv ‘Rainbow. According to SEM and TEM analysis, synthesized NUs remained within size of 25.36 ± 12.55 nm length and 16.18 ± 3.75 nm width exhibiti ng a polygonal shape. In well diff usion test, opti mized 30 ppm concentrati on of Cu NUs formed an inhibiti on zone with a diameter of 20.6 ± 0.94 mm. In comparison, the anti bioti c sulfamethoxazole (positi ve control) formed an inhibiti on zone with a diameter of 26.3 ± 0.47 mm. At in-vivo level, 200, 100 and 50 ppm all three concentrati ons were able to control disease in 90% of treated plants within 5, 6 and 7 weeks respecti vely. Overall, this research highlights the potenti al of Cu NUs as an eff ecti ve alternati ve for controlling bacterial blight disease in anthurium cv ‘Rainbow’.