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Publication Open Access Copper-Based Nanoparticles as Precision Nanoantibiotics against Bacterial Blight in Anthurium andraeanum Lind.: Synthesis, Characterization, and Antibacterial Efficacy under In Vitro and In Vivo Conditions(American Chemical Society, 2026-05-05) Wanaguru, S; Jayasinghe, S; Seneviratne, K; Kottegoda, N; Peiris, Sriyani E; Peiris, Colin NThe bacterial blight disease caused by Xanthomonas spp., a dilemma for farmers worldwide. The existing conventional methods have failed to effectively combat the disease. Therefore, it is crucial to focus on developing and implementing more affordable techniques to effectively manage bacterial blight effectively. Herein, we investigated the efficacy of copper-based nanoparticles (Cu NPs) synthesized via three different methods toward controlling bacterial blight disease in Anthurium andraeanum plants. For that, Cu NPs were engineered via chemical, semichemical, and green synthesis approaches. Cu NPs were characterized through scanning electron microscope (SEM), transmission electron microscope (TEM), Fourier-transform infrared (FTIR), and powder X-ray diffraction (PXRD) analysis. Quantitative antibacterial assays revealed that the chemical and green-synthesized Cu NPs exhibited the highest potency, with a minimum inhibitory concentration (MIC) of 31.25 μg/mL and a minimum bactericidal concentration (MBC) of 62.50 μg/mL, whereas the semichemical formulation showed lower activity (MIC = 62.50 μg/mL; MBC = 125 μg/mL). In vitro well-diffusion assays further supported this trend: at 30 ppm, the inhibition zones measured 25 ± 0.81, 15 ± 0.81, and 20.6 ± 0.94 mm for the chemical, semichemical, and green synthesis methods, respectively, compared to 26.3 ± 0.47 mm for the positive control-antibiotic sulfamethoxazole. At in vivo, optimized concentration of 200 ppm, Cu NPs produced via all three methods controlled bacterial blight disease in 90% of treated anthurium plants within 4, 7, and 5 weeks, respectively, without causing phytotoxicity. Disease progression was monitored over 12 weeks by measuring lesion areas, and statistical significance of treatments was determined using two-way analysis of variance (ANOVA). ©Publication Open Access Transient expression of uida reporter gene in regenerable callus tissues of Anthurium andraeanum lind. by agrobacterium mediated transformation(Postgraduate Institute of Agriculture, University of Peradeniya: Peradeniya, 2000) Yakandawala, G; Peiris, S; Yakandawala, Y. L. NA tissue culture scheme for plant regeneration via callus, and a protocol for gene transfer to regenerable callus are described for Anthurium andraeanum variety A vo Nette. Callus was induced from shoot bases and from leaf blades of Anthurium andraeanum on modified MS media containing 0.3 mg t' 2,4-D and 0.5 mg t' BAP. Shoot bases, leaf blades with a single cut at the base, and sectioned leaf blades produced callus at frequencies of 90%, 20% and 8%, respectively. Plant regeneration was obtained at a high frequency in all callus types, irrespective of their origin, in modified MS media containing 0.5 mg t' BAP. Callus was tested for gene transfer efficiency by 2 Agrobacterium tumefaciens strains, C58 andLB4404, carrying binary vector p CAMBIAi } oi containing hpt and uidA reporter genes under plant expression signals. Transient expression of uidA gene monitored by GUS histochemical assay was observed only in callus inoculated with Agrobacterium strain LB4404 at a frequency of 3.33%.