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Author: search.filters.author.Wijenayake, U

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    PublicationOpen Access
    Anthocyanin (ATH)-incorporating polyvinylpyrrolidone-ethyl cellulose-(2-hydroxypropyl)-β-cyclodextrin (PVP–EC–BCD) nanofiber-based pH sensor for ocular pH detection during accidental chemical spills
    (Royal Society of Chemistry, 2026-02-03) Sandaruwan, B; Liyanage, R; Costha, P; Dassanayake, R.S; Wijesinghe, R.E; Herath H.M.L.P.B.; Nalin de S.K.M; de Silva, R.M; Rajapaksha, S.M; Wijenayake, U; Manatunga, D.C
    The existing ocular pH detection methods encounter numerous limitations, including low accuracy, poor sensitivity across a wide pH range, and patient discomfort, highlighting the need for innovative approaches. A novel biosensor for ocular pH detection has been developed to assess ocular health and chemical injuries in clinical settings. This study uses the pH-sensitive properties of anthocyanins (ATHs), natural pigments extracted from butterfly pea flowers, to develop a novel pH-responsive nanofiber mat. ATHs are integrated into a polymer blend containing polyvinylpyrrolidone (PVP), ethyl cellulose (EC), and (2-hydroxypropyl)-β-cyclodextrin (BCD) to fabricate electrospun nanofibers. The acquired characterization, employing scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA), confirmed the successful fabrication of the ATH-infused nanofibers with a mean diameter ranging from 121 to 396 nm. Four formulations were tested: PVP:EC:BCD:ATH (18 ppm), PVP:EC:BCD:ATH (25 ppm), PVP:EC:BCD:ATH (35 ppm), and PVP:EC:BCD:ATH (50 ppm). Among them, the 50 ppm ATH-incorporating nanofiber mat exhibited the best performance in terms of color clarity, response time, and pH sensitivity. The fabricated 50 ppm ATH incorporating nanofiber mat demonstrated a rapid pH response time of less than 5 seconds (s) while exhibiting a color variation from pink to blue to green across the pH range of 1 to 12, providing a rapid and accurate method for visual pH detection. Based on the color performance of the 50 ppm ATH-incorporating system, a standardized color reference chart was developed to serve as a practical and visual guide for estimating pH levels in clinical applications. Zebrafish toxicity assays were conducted further to validate the safety and biocompatibility of the developed sensor, revealing no significant toxic effects across the range of ATH concentrations.
  • Thumbnail Image
    PublicationEmbargo
    High-resolution optical imaging for sustainable fish freshness and safety assessment
    (Elsevier GmbH, 2026-04) Madhubhashini, M. N; Kahandawala, B.S; Sandaruwan, H.H.P. B; Silva, B.N; Wijenayake, U; Wijesinghe, R.E
    Fish freshness evaluation is crucial to ensure consumer safety, and rapid assessment is essential for effective and accurate quality control. To overcome the limitations of the gold standards, such as lack of structural depth information, high-time consumption, and labor-intensiveness, high-resolution Optical Coherence Tomography (OCT) was employed for real-time monitoring of fish freshness non-invasively. Microstructural changes of eye and skin of Indian Anchovies ( Stolephorus indicus ) specimens were considered as the main freshness parameters during refrigeration storage. Both eye and skin tissues exhibited decreased internal scattering, loss of clarity, boundary weakening, and gradual structural degradations through the OCT observations. The quantitatively assessed variance intensity, entropy, energy, and edge density clearly revealed the internal tissue disruption over storage time due to protein denaturation, oxidative damage, and fluid imbalance. The findings of this study indicate that OCT shows an insightful correlation with microbiological and biochemical spoilage processes, enabling the advanced identification of subtle microstructural changes in fish skin and eye, even at a prior stage of deterioration. Such capability offers an objective and rapid freshness evaluation approach that could greatly benefit supply chain management and post-harvest seafood quality monitoring.
  • Thumbnail Image
    PublicationOpen Access
    Anthocyanin (ATH)-incorporating polyvinylpyrrolidone-ethyl cellulose-(2-hydroxypropyl)-β-cyclodextrin (PVP–EC–BCD) nanofiber-based pH sensor for ocular pH detection during accidental chemical spills
    (Royal Society of Chemistry, 2026-02-03) Sandaruwan, B; Liyanage, R; Costha, P; Dassanayake, R.S; Wijesinghe, R.E; Herath H.M.L.P.B; Nalin de S.K.M; de Silva, R.M; Rajapaksha, S.M; Wijenayake, U
    The existing ocular pH detection methods encounter numerous limitations, including low accuracy, poor sensitivity across a wide pH range, and patient discomfort, highlighting the need for innovative approaches. A novel biosensor for ocular pH detection has been developed to assess ocular health and chemical injuries in clinical settings. This study uses the pH-sensitive properties of anthocyanins (ATHs), natural pigments extracted from butterfly pea flowers, to develop a novel pH-responsive nanofiber mat. ATHs are integrated into a polymer blend containing polyvinylpyrrolidone (PVP), ethyl cellulose (EC), and (2-hydroxypropyl)-β-cyclodextrin (BCD) to fabricate electrospun nanofibers. The acquired characterization, employing scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA), confirmed the successful fabrication of the ATH-infused nanofibers with a mean diameter ranging from 121 to 396 nm. Four formulations were tested: PVP:EC:BCD:ATH (18 ppm), PVP:EC:BCD:ATH (25 ppm), PVP:EC:BCD:ATH (35 ppm), and PVP:EC:BCD:ATH (50 ppm). Among them, the 50 ppm ATH-incorporating nanofiber mat exhibited the best performance in terms of color clarity, response time, and pH sensitivity. The fabricated 50 ppm ATH incorporating nanofiber mat demonstrated a rapid pH response time of less than 5 seconds (s) while exhibiting a color variation from pink to blue to green across the pH range of 1 to 12, providing a rapid and accurate method for visual pH detection. Based on the color performance of the 50 ppm ATH-incorporating system, a standardized color reference chart was developed to serve as a practical and visual guide for estimating pH levels in clinical applications. Zebrafish toxicity assays were conducted further to validate the safety and biocompatibility of the developed sensor, revealing no significant toxic effects across the range of ATH concentrations.