Correlation between molecular diversity and biochemical traits of edible aerial parts of Basella alba L. from different geographical locations of Sri Lanka

Abstract

Background: Basella alba L. a widely consumed green leafy vegetable, exhibits considerable nutritional and therapeutic potential attributed to its bioactive constituents. Prior investigations revealed significant variation in phytochemical and antioxidant activity across agro-climatic zones in Sri Lanka, suggesting potential genetic influence. This study is designed to explore underlying genetic variation using RAPD markers to investigate the correlations and contributions of genotype on previously reported bioactivity variation. Results: From a screening of 15 RAPD primers, four primers (OPA 9, OPA 10, OPA 16, and OPB 10) produced, polymorphic, consistent and clearly scorable banding profiles (under optimized PCR conditions) in B. alba L. collected from 15 Sri Lankan locations. These primers collectively yielded 36 bands, 35 of which were polymorphic, resulting in a high polymorphism rate of 97.2%, confirming the informativeness of the selected primers for genetic diversity analysis. Genetic similarity was assessed using Jaccard’s coefficient in NTSYSpc.v2·10e, revealing values ranging from 0.44 to 0.97, with the highest similarity from the samples from Ratnapura and Kandy and the lowest similarity in Ratnapura and Kalutara. A dendrogram constructed via UPGMA grouped the samples into two major clusters and five sub-clusters, demonstrating substantial genetic differentiation influenced by geographic origin. Cluster I included Ratnapura and Kandy, while the remaining samples formed Cluster II and its subgroups, each representing different ecological zones. When compared to the phytochemical and antioxidant clustering data of the previous study, partial correspondence was observed. A Mantel test comparing genetic diversity and biochemical/antioxidant potential revealed a weak negative correlation which was not significantly different. Some of the locations within similar genetic cluster shared similar biochemical traits, while others diverged significantly, indicating that environmental conditions also influence bioactive compound synthesis. Notably, Cluster I (Ratnapura and Kandy) showed both genetic similarity and lower antioxidant traits. Samples from Ella and Polonnaruwa showed similar bioactive traits even though they were grouped into different genetic clusters. Conclusion: These findings suggest that both genetic makeup and environmental adaptation contribute to observed biochemical diversity in B. alba L. with a clear geographical correlation. This study highlights the value of integrating molecular and biochemical analyses to develop regionally adapted B. alba L. cultivars with enhanced nutritional traits, supporting sustainable agriculture in Sri Lanka and beyond.