Research Papers - School of Natural Sciences

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Subject: search.filters.subject.Micropropagation

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Now showing 1 - 3 of 3
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    Plant Regeneration System for Osbeckiaoctandra (L.) DC: A Valuable Medicinal Plant
    (Sri Lanka Institute of Information Technology, 2020-12-01) Nagahatenna, D. S. K; Peiris, S
    Osbeckiaoctandra (HeenBovitiya), which is one of the most valuable ayurvedic medicinal and ornamental plants in Sri Lanka, is now threatened due to its overexploitation from their natural habitat. In order to produce high quality, disease-free and genetically identical plant materials in large scale, we developed a highly efficient in vitro clonal propagation system using leaf explants. The effects of three different concentrations of plant growth regulators (6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA), kinetin and incubation conditions on plant regeneration were investigated. Plant growth parameters were analyzed in 15 biological replicates using one-way ANOVA. Present study revealed that the highest number of shoots per leaf explant with 92.4% shoot induction rate was achieved when young mature leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 3 mg/L BAP and 0.5 mg/L NAA and incubated under dark conditions. When microshoots were separated and subcultured onto hormone-free MS medium containing high sugar content (4%), rapid shoot multiplication and a vigorous root development was detected under light conditions. The in vitro grown plantlets were successfully acclimatized and 89% of the regenerated plantlets survived. Our novel clonal propagation system will open new avenues for mass propagation of O. octandra plants for the pharmaceutical industry and improving their medicinal and ornamental characteristics through biotechnological tools
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    An Efficient Plant Regeneration Using Leaf Explant for Osbeckia octandra (L.) DC; A Valuable Medicinal and Ornamental Plant
    (Faculty of Humanities and Sciences - SLIIT, 2021-03-26) Dilrukshi, S. K.; Nagahatenna, D.S.K.; Peiris, Sriyani E.
    Osbeckia octandra (Heen Bovitiya), which is one of the most valuable ayurvedic medicinal and ornamental plants in Sri Lanka is now threatened due to overexploitation in its natural habitat. In order to preserve this economically important plant species, a highly efficient in vitro clonal propagation system using leaf explants was developed in this study. The effects of three different concentrations of plant growth regulators: (6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA) and kinetin) and incubation conditions on plant regeneration were investigated. The plant growth parameters were analyzed in 15 biological replicates using one- way or two-way ANOVA. The present study revealed that the highest number of shoots per leaf explant with 92.4% shoot induction rate was achieved when young mature leaf explants were inoculated on Murashige and Skoog (MS) medium supplemented with 3 mg/L BAP and 0.5 mg/L NAA under dark conditions. When micro shoots were separated and subcultured on hormone-free MS medium with high sugar content (4%), shoots multiplied rapidly and a vigorous root system was developed under light conditions. The in vitro grown plantlets were successfully acclimatized and 89% of the regenerated plantlets survived. Our novel clonal propagation system will open new avenues for large- scale multiplication and improvement of medicinal and ornamental characteristics of this valuable species using biotechnological tools.
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    Production of Disease Free Rumohra adiantiformis (Leatherleaf Fern) Using In Vitro Propagation
    (Faculty of Humanities and Sciences - SLIIT, 2021-03-26) Perera, D.; Peiris, S.E.
    This study investigated the use of rhizome tips as explants in regenerating disease-free plantlets of Rumohra adiantiformis (G. Frost) using the micropropagation technique. Rhizome tips of 0.5 cm, 1 cm, 1.5 cm, and 2 cm in length, and with and without outer skin were used as explants.The highest regeneration rate (30%) was achieved with an explant size of one centimetre when the outer skin was not present in rhizome explants. Rhizome tips produced complete plantlets in Murashige and Skoog (MS) medium without any growth regulators in 80 - 90 days of culture initiation. In vitro multiplication of disease-free plants was achieved by culturing four months old in vitro plantlets in the MS medium supplemented with 2 mg/L Benzyl Amino Purine (BAP) and 0.1mg/L Naphthalene Acetic Acid (NAA). Plantlets produced clusters of shoot primordia which are known as green globular bodies (GGB) in the multiplication medium, 10 weeks after culturing. Plantlets were obtained by culturing 2-3 mm size GGB segments in the basal medium without any plant growth regulators. Disease indexing of the in vitro derived plantlets verified that at in vitro level 60% of the plantlets obtained from the rhizome tips of one centimetre were free from bacterial and fungal diseases.