In vitro propagation of Hemidesmus indicus (L) R. Br.(Iramusu) through nodal culture

Abstract

he effects of different nodal positions and 6-benzylaminopurine (BAP) concentrations on shoot development from nodal explants of Hemidesmus indicus (L.) Br.(Iramusu) were investigated. The highest number of shoots per explant (2.57±0.97) was observed in 4th nodal position, while the longest shoot length (3.23±0.52 cm) was achieved in 5th nodal position at 2 mg/L BAP with 0.1 mg/L Naphthalene Acetic Acid (NAA). Reduction of total shoot length and number of shoots were observed when BAP concentration was gradually increased from 5 to 15 mg/L. Half-strength semi solid MS medium with 1.5 mg/L indole-3-butyric acid (IBA) exhibited the best in vitro rooting. Hundred percent of the rooted shoots survived during the acclimatization.